Thermo Fisher Scientific Cyclin B1 Polyclonal Antibody
Cyclin B1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 Affinity Chromatography 정제, -20°C 보관. 세포주기 G2/M 전이 연구용으로 이상적.
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Thermo Fisher Scientific Cyclin B1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 334–433 of human Cyclin B1 (NP_114172.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.05 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913990 |
Product Specific Information
Positive test controls include: C2C12, C6.
Localization: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Immunogen sequence:
YDMVHFPPSQ IAAGAFCLAL KILDNGEWTP TLQHYLSYTE ESLLPVMQHL AKNVVMVNQG LTKHMTVKNK YATSKHAKIS TLPQLNSALV QDLAKAVAKV
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for cell cycle control at the G2/M transition. It forms a complex with p34 (cdc2) to create the maturation-promoting factor (MPF).
Two alternative transcripts exist: one constitutive and one cell cycle-regulated, expressed predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and is destroyed at the end of mitosis via APC-dependent degradation. Overexpression is observed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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