Thermo Fisher Scientific Mouse IgG2a/IgG2b Isotype Control Cocktail, PE-Cyanine5

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Flow cytometry용 마우스 IgG2a/IgG2b 동형 대조 항체 칵테일. 비특이적 결합 평가에 사용. PE-Cyanine5 형광 표지로 568/666 nm에서 발광. 액상 형태로 4°C 보관, 동결 금지.

카탈로그번호: SA112477
브랜드: Thermo Fisher Scientific
카테고리: Monoclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific SA112477 Mouse IgG2a/IgG2b Isotype Control Cocktail, PE-Cyanine5 100 tests pk판매 단위 pk ·

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Thermo Fisher Scientific · Thermo Fisher Scientific Mouse IgG2a/IgG2b Isotype Control Cocktail, PE-Cyanine5

Applications

Flow Cytometry (Flow): Assay-dependent
Control (Ctrl): Assay-dependent

Product Specifications

항목	내용
Host/Isotype	Mouse / IgG2a/IgG2b
Class	Cocktail
Type	Isotype Control
Clone	ZX4
Immunogen	White blood cells
Conjugate	PE-Cyanine5
Excitation/Emission Max	568/666 nm
Form	Liquid
Storage conditions	4°C, store in dark, DO NOT FREEZE!
Shipping conditions	Wet ice
RRID	AB_1077921

Product Specific Information

This antibody contains a mix of IgG2a (Cy5) and IgG2b (RPE) isotypes.
SA1-12477 has been successfully used in FACS procedures to assess non-specific binding of mouse monoclonal antibodies in human samples.

Target Information

The isotype of a primary antibody and the application it is being used in can result in background staining.
Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence.

Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.
While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts.

Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined.
If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.