Thermo Fisher Scientific PLK4 Monoclonal Antibody (OTI3G10), TrueMAB
PLK4 단백질을 인식하는 마우스 단클론 항체로, Western blot에 최적화되어 있습니다. Human PLK4(aa 307-612)에서 유래한 재조합 단백질로 면역화되었습니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 307–612 of human PLK4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
PLK4 (Polo-like kinase 4) is a serine/threonine protein kinase localized to centrioles, regulating centriole duplication during the cell cycle.
Overexpression of PLK4 induces formation of multiple procentrioles, leading to centriole amplification and biogenesis in human cells.
Reduced PLK4 gene dosage increases the likelihood of mitotic errors and cancer development.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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