Thermo Fisher Scientific ACOT2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (IHC)

1:100-1:300

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

ELISA (ELISA)

1:40,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthesized peptide derived from the Internal region of human ACOT2 (aa 171-220). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ACOT2,uniProtId:P49753-1,ncbiNodeId:9606,antigenRange:171-220,antigenLength:483,antigenImageFileName:PA5-50905_ACOT2_P49753-1_Rabbit.svg,antigenImageFileNamePDP:PA5-50905_ACOT2_P49753-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.5% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2636353

Product Specific Information

ACOT2 Polyclonal Antibody detects endogenous levels of ACOT2 protein.

Target Information

Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of acyl-CoA to form coenzyme A (CoA) and a free fatty acid. Through their catalytic activity, ACOTs are able to regulate the level of fatty acids and acyl-CoAs within the cell. ACOT1 (acyl-CoA thioesterase 1, also known as CTE1) and ACOT2 (acyl-CoA thioesterase 2, also known as PTE2) are members of the ACOT family and exhibit different cellular localization, with ACOT1 existing as a monomer in the cytoplasm and ACOT2 localized primarily to mitochondria. Characteristic of most ACOT proteins, ACOT1 and ACOT2 catalyze the conversion of Palmitoyl-CoA and water to free CoA and palmitate, a reaction that is important for the regulation of intercellular fatty acid levels. ACOT2 is expressed as multiple alternatively spliced isoforms and, like ACOT1, is encoded by a gene which maps to human chromosome 14.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.