Thermo Fisher Scientific Precision ID Identity Panel

Applied Biosystems™ Precision ID Identity Panel

The Applied Biosystems Precision ID Identity Panel is one of the first next-generation sequencing (NGS) solutions for human identification that uniquely identifies pristine or degraded samples.
The multiplexed panel of SNPs provides discrimination of individuals similar to STR genotype match probabilities used by forensic analysts (between 1×10⁻³¹ and 6×10⁻³⁵).

High discrimination power is achieved by using 34 upper Y-Clade SNPs and 90 autosomal SNPs with high heterozygosity and low fixation index (Fst), as described by Dr. Kenneth Kidd (Yale University) and the SNPForID Consortium.
Optimized for degraded or challenging forensic samples, the panel requires only 1 ng of DNA input, with acceptable performance down to 100 pg.
Average amplicon lengths: 132 nucleotides (autosomal markers) and 141 nucleotides (Y-markers).
Using the Precision ID NGS System and Converge Software, genotypes can be obtained in less than two days.

Key Features

Simplicity

• Small amplicons enable better recovery with degraded samples
• Works with as little as 100 pg of DNA input
• Automated library and template preparation using the Ion Chef System

Scalability

• Multiple chip formats for various sample throughput requirements
• Multiplexing capability of up to 384 samples*

Speed

• Less than 45 minutes of hands-on time for DNA-to-data workflow*
• Total sample-to-data time under 2 days*

*Using the Precision ID NGS System for Human Identification

Kit Composition

Provides sufficient material for:

• 96 reactions using the manual Precision ID Library Kit (Cat. No. A26435)
• 32 reactions using the automated Precision ID DL8 Library Kit (Cat. No. A33212)

References

1. Pakstis, AJ et al. (2010) Human Genetics 127(3), 315–324.
2. Phillips, C et al. (2007) Forensic Science International: Genetics 1(2), 180–185.
3. Karafet, TM et al. (2008) Genome Research 18(5), 830–838.

사양 (Specifications)

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