Invitrogen™

EXPRESS One-Step Superscript™ qRT-PCR Kit, universal

EXPRESS One-Step SuperScript qRT-PCR kits incorporate proven Invitrogen enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for자세히 알아보기

EXPRESS One-Step SuperScript qRT-PCR kits incorporate proven Invitrogen enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments. This fast, one-step mix is designed to work with a variety of instruments` default protocols, including Applied Biosystems and non-Applied Biosystem (see universal version) qPCR platforms.

Features of the EXPRESS One-Step Superscript qRT-PCR Kit include:
• Fast-activating, antibody-mediated Platinum Taq DNA Polymerase–complete, rapid activation; provides high sensitivity and reproducibility
• The One-Step SuperScript III Reverse Transcriptase module– a unique formulation to ensure high yield and less PCR inhibition
• Flexible formats with separate or pre-mixed ROX–designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler™ 480, Corbett Rotor-Gene™)
• Contains heat-labile UDG–significantly reduces carryover contamination risk with excellent activity from room temperature to approximately 40°C. Due to the thermostability of SuperScript III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated

EXPRESS kits work with manufacturer`s default protocols on fast instruments and come in a convenient, stable SuperMix format for ultimate convenience. The kits are offered with either pre-mixed ROX or a separate tube of ROX to fit your instrument needs, as well as SYBR Green chemistry options:
EXPRESS One-Step Superscript qRT-PCR Kit, universal (this page)
EXPRESS One-Step Superscript qRT-PCR Kit, with premixed ROX
EXPRESS One-Step SYBR GreenER Kit, with premixed ROX
EXPRESS One-Step SYBR GreenER Kit, universal

사양

검출 방법Primer-probe

형식Tube

PCR 방법1-step RT-qPCR

중합효소Taq DNA Polymerase

반응 속도Fast or Standard

용도(장비)7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ

제품라인SuperScript™

제품 유형qRT-PCR Kit

수량500 reactions

충분500 Reactions

반응 수500 Reactions

샘플 종류RNA

Unit SizeEach