Thermo Fisher Scientific MAP2 Monoclonal Antibody (AP20)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20-1:50

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:200

View 17 publications 17 publications

Product Specifications

Species Reactivity

Bovine, Human

Published species

Human, Mouse, Non-human primate, Rat, Xenopus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AP20

Immunogen

Bovine brain microtubule associated protein 2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10982160

Product Specific Information

This antibody is specific to MAP2a and MAP2b, and will not cross-react with MAP1, MAP5, tubulin or tau. This clone reacts with dendrites and cell bodies of neurons but not with the neuronal processes. It does not react with the low MW form (70 kDa, MAP2c). The epitope has been mapped to aa 997-1332.

The molecular weight of the doublet is ~280 kDa.

For IHC staining, formalin-fixed tissues must be boiled in a 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Recommened positive controls include human glioblastoma T98G cells or brain tissue.

Target Information

MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer`s Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.