Gibco™

MEM α, nucleosides, no ascorbic acid

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative자세히 알아보기

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With	Without
Deoxyribonucleosides	Ascorbic acid
Ribonucleosides
L-glutamine
Phenol Red

The complete formulation is available.

Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This MEM is modified without ascorbic acid. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

사양

세포주HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

세포 유형Primary Rat Astrocytes

분류Animal Origin-free

농도1 X

형태Liquid

제품 유형MEM α (Minimum Essential Medium α)

Serum LevelStandard Serum Supplementation

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

첨가제 포함Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Deoxyribonucleosides, Ribonucleosides

첨가제 없음No Ascorbic Acid

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach