
Thermo Fisher Scientific MEM α, nucleosides, no ascorbic acid
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Gibco™
MEM α, nucleosides, no ascorbic acid
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative자세히 알아보기
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
With | Without |
Deoxyribonucleosides | Ascorbic acid |
Ribonucleosides | |
L-glutamine | |
Phenol Red |
The complete formulation is available.
Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This MEM is modified without ascorbic acid. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
사양
세포주HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
세포 유형Primary Rat Astrocytes
분류Animal Origin-free
농도1 X
형태Liquid
제품 유형MEM α (Minimum Essential Medium α)
Serum LevelStandard Serum Supplementation
유통 기한12 Months From Date of Manufacture
멸균Sterile-filtered
첨가제 포함Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Deoxyribonucleosides, Ribonucleosides
첨가제 없음No Ascorbic Acid
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco™
수량500 mL
배송 조건Room Temperature
Unit SizeEach
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