
Thermo Fisher Scientific Goat anti-Mouse IgM Cross-Adsorbed Secondary Antibody, DyLight 488
DyLight 488 형광 표지를 가진 염소 유래 항-마우스 IgM 2차 항체로, 인간 및 랫트에 대한 교차 반응 최소화. Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 높은 특이성과 감도로 신호 증폭 및 다양한 검출 시스템에 적합.
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Thermo Fisher Scientific Goat anti-Mouse IgM Cross-Adsorbed Secondary Antibody, DyLight 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgM |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 6.8–7.4) with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556730 |
| Target | IgM |
| Cross Adsorption | Against human and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to human and rat.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target molecules. Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification. Typically, they are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- Custom conjugation available upon request
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