Invitrogen™

Ambion™ In Vivo GAPDH Positive Control siRNA

Ambion™ In Vivo siRNA ControlsValidated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcriptsPositive Control siRNAs functionally tested자세히 알아보기

Ambion™ In Vivo siRNA Controls

• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer™ Select modifications for enhanced specificity or Ambion™ In Vivo modifications for increased stability
• For use in human, mouse, and rat cells

What are Ambion™ In Vivo siRNAs?

Ambion™ In Vivo siRNAs are designed using the proven Silencer_™ Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion™ _In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion™ In Vivo siRNAs exhibit equivalent or better potency than _Silencer_™ Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.

GAPDH Positive Control siRNAs

Our extensively validated, positive control siRNA to human, mouse, and rat GAPDH serves multiple functions. First, it is an ideal test siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. In addition, because it targets GAPDH mRNA, which is commonly used as an internal control, its effects are easy to assay, and thus provides an excellent tool to monitor siRNA transfection efficiency by real-time RT-PCR. The Ambion™ In Vivo GAPDH Positive Control siRNA shows increased serum and nuclease resistance compared with _Silencer_™ Select siRNAs without loss of potency or specificity and is recommended for in vivo applications.

Quality Control

Ambion™ synthesizes and purifies each Ambion™ In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

In Vivo Ready Controls

Our In Vivo Ready Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 μM in presence of deionized water, in vivo Ready siRNAs contain less than 5.0 mM Na+, less than 0.06 mM K+, and less than 0.02 mM Mg2+.

사양

제어 유형Positive Control

RNAi TypesiRNA

용도(애플리케이션)RNAi, Transfection

라벨 또는 염료Unconjugated

제품라인Ambion™

제품 유형siRNA

순도In Vivo, HPLC

수량5 nmol

배송 조건Room Temperature

TargetGAPDH

Unit SizeEach