Thermo Fisher Scientific Laminin Polyclonal Antibody, DyLight 550

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

-

Immunohistochemistry (Frozen) (IHC (F))

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

Assay-Dependent

-

Product Specifications

Species Reactivity

Hamster, Human, Invertebrate, Mammal, Mouse, Sheep, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

DyLight™ 550 DyLight™ 550 DyLight™ 550

Excitation/Emission Max

556/570 nm View spectra

Form

Liquid

Concentration

0.99 mg/mL

Purification

IgG fraction

Storage buffer

50mM sodium borate

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11157672

Product Specific Information

This antibody is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).

The antibody functionally inhibits Laminin in mouse and rat. It binds to Laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue. Suggested positive control: basement membranes (blood vessels of any tissue, tumors, nerve).

Target Information

Laminin antibodies are widely used to label blood vessels and basement membranes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.