Thermo Fisher Scientific DCAF4 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.2–1 µg/mL

Product Specifications

Product Specific Information

• Immunogen sequence:
  GHRQSFGTNS DVLAQQFALM APLLFNGCRS GEIFAIDLRC GNQGKGWKAT

• Storage Guidance:
  For short term use, store at 2–8°C up to 1 week.
  For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

• Predicted Homology:
  Cow: 86%
  Dog: 93%
  Guinea Pig: 86%
  Horse: 93%
  Human: 100%
  Mouse: 79%
  Pig: 100%
  Rabbit: 93%
  Rat: 86%

Target Information

The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M. It is exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
During mitosis, it is strictly associated with the spindle pole and mitotic spindle, whereas during S and G2 phases, it is diffusely distributed throughout the nucleus.
The full-length cDNA encodes a 747-amino acid protein with a putative ATP/GTP-binding site motif.
RT-PCR analysis demonstrated strong expression in lung carcinoma cell lines but not in normal lung tissues. Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine, and in fetal brain, liver, and kidney.
P100 is also correlated to cancer prognosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.