Thermo Fisher Scientific PPAT Monoclonal Antibody (OTI1B8), TrueMAB
PPAT 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 IHC(P)에서 검증됨. Human, Mouse, Rat, Dog 반응성. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 퓨린 생합성 경로 연구용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 42–278 of human PPAT produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The protein encoded by the PPAT gene belongs to the purine/pyrimidine phosphoribosyltransferase family.
It is a regulatory allosteric enzyme catalyzing the first step of de novo purine nucleotide biosynthesis.
This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine biosynthesis pathway, are located in close proximity on chromosome 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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