Thermo Scientific™

GeneBLAzer™ RXRα-UAS-bla HEK 293T Cells

RXR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoid X receptor-alpha (RXR alpha) fused to자세히 알아보기

RXR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoid X receptor-alpha (RXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UASbla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of alpha-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. RXR alpha-UAS-bla HEK 293T cells are functionally validated for Z` and EC50 concentrations of 9-cis-retinoic acid. In addition, RXR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

사양

대체 작용제Dexamethasone, Progesterone, Cortisol

길항제Spironolactone

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Dividing Cells

검출 방법Fluorescence

종Human

약효를 기대할 수 있는 타겟Nuclear Receptors

셀 수2 x 106 Cells

일차 작용제Aldosterone

제품라인GeneBLAzer™

제품 유형HEK 293T Cells

수량2 x 106 cells

리포터 유전자BLA (Beta-Lactamase)

타겟 입력RXRA, RXR alpha, NR2B1

Unit SizeEach