Thermo Fisher Scientific TaqMan GAPDH Assay, JUN dye/QSY probe, primer-limited

Applied Biosystems™ TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe, primer-limited

The Applied Biosystems™ TaqMan™ GAPDH Assay with JUN™ dye-labeled QSY™ probe (primer-limited) provides a pre-formulated assay for quantitating human GAPDH.
This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of a JUN™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers.
The assay can be used for multiplex or singleplex PCR reactions, and it is primer-limited, allowing use with samples that overexpress GAPDH.

주요 특징

• 프리디자인된 프라이머 및 JUN™ dye-labeled TaqMan™ probe로 실험 설계 시간 절감
• 실험 최적화 시간 최소화
• FAM™ 또는 VIC™ dye-labeled gene expression assay와 병용 가능
• 멀티플렉스 반응용 프라이머 제한 농도로 조제

Save time in assay design, formulation, and testing by using the TaqMan™ GAPDH Assay as your control when quantifying gene expression.
This pre-designed probe and primer set enables normalization of sample RNA or DNA amounts when used with a control sample.
It can also be used for relative gene expression quantification in cDNA samples when used with other gene expression assays.

For multiplex experiments, the primer-limited formulation allows use with high GAPDH expressors.
The JUN™ dye is optimized for use with the 4th filter of QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments.
Note: Master mix containing ROX™ dye cannot be used with this assay due to spectral overlap. TaqMan™ Multiplex Master Mix with MUSTANG PURPLE™ dye is recommended.

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