Applied Biosystems™

TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe, primer-limited

The Applied Biosystems™ TaqMan™ GAPDH Assay with JUN™ dye-labeled QSY™ probe (primer limited) provides a pre-formulated assay for quantitating human자세히 알아보기

The Applied Biosystems™ TaqMan™ GAPDH Assay with JUN™ dye-labeled QSY™ probe (primer limited) provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of a JUN™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers. The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress GAPDH.

Benefits of this assay include:

• Eliminate assay design time by using pre-designed primers and pre-designed JUN™ dye-labeled TaqMan™ probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assays
• Formulated at a primer-limited concentration for use in multiplex reactions

Save time in assay design, formulation, and testing by using the TaqMan™ GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high GAPDH expressors.

The JUN™ dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN™ dye) due to an overlap of spectra. TaqMan™ Multiplex Master Mix with MUSTANG PURPLE™ dye is recommended.

사양

등록 번호NM_002046.3

앰플리콘 크기118

검출 방법Primer-probe

형식Tube

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

유전자 기호GAPDH

그린 기능Sustainable packaging

내부 프로브 수정QSY™ Non-Fluorescent Quencher (3`)

라벨 또는 염료JUN

제품라인TaqMan™

제품 유형Gapdh Assay

수량250 reactions

종Human

충분250 Reactions

타겟Glyceraldehyde-3-phosphate dehydrogenase

용도(애플리케이션)Gene Expression

반응 수250 Reactions

Unit SizeEach