Thermo Fisher Scientific Calcineurin B Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:20–1:50

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence: GRRFKKLDLD KSGSLSVEEF MSLPE

Target Information

Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits:

• Calcineurin A (CnA): 60 kDa catalytic subunit, highly homologous to protein phosphatases 1 and 2A
• Calcineurin B (CnB): 19 kDa regulatory subunit containing four EF-hand calcium binding sites

Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. The immunosuppressive drugs cyclosporin A and FK506, when bound to cyclophilin and FKBP respectively, inhibit NF-AT activation by blocking substrate access to the catalytic domain of CnA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.