Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Yellow 700, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Yellow 700, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV/Violet/Yellow series
• NovaFluor Blue/Red/UV/Violet/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series
• Custom conjugation available

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with the B cell receptor (BCR). Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation. The SJ25C1 and HIB19 antibodies recognize overlapping epitopes.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use at 5 µL (0.4 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

Note: NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.

This NovaFluor conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which provides formulation improvements over the original CellBlox. Use 5 µL of CellBlox Plus Blocking Buffer per sample (regardless of number of NovaFluors). For single-color controls, use 5 µL per 100 µL of cell sample containing 10³–10⁸ cells.

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures engineered for optimized fluorescent signatures and reduced spillover/spread.

Excitation: 552 nm
Emission: 700 nm
Laser: 561 nm (Yellow)

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and may appear on myeloid leukemia cells. CD19 is the earliest and most broadly expressed B cell-restricted antigen, regulating normal and malignant B cell proliferation. It lowers the threshold for antigen receptor-dependent stimulation by forming complexes with BCR. CD19 can also signal independently and serves as a central regulator of multiple signaling pathways. Mutations cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지