Thermo Fisher Scientific CD103 Monoclonal Antibody (LF61), TRI-COLOR

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LF61

Immunogen

Human CD103

Conjugate

TRI-COLOR™ TRI-COLOR™ TRI-COLOR™

View additional formats

• FITC
• PE
• Request custom conjugation

Excitation/Emission Max

567/671 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with sucrose

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372346

Product Specific Information

The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5 conjugate that permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

Target Information

CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

Analyte Specific Reagent