Thermo Fisher Scientific ATF6 Monoclonal Antibody (3D5)
ATF6 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot, IHC, ELISA에 적합합니다. 인간 시료 반응성이 있으며, ER 스트레스 관련 단백질 연구에 유용합니다. 액상 형태로 제공되며 PBS buffer에 보존됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D5 |
| Immunogen | ATF6 (AAH14969.1, 91 a.a. approximately 193 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
QPLSPASSSY SVSSPRSVDS YSSTQHVPEE LDLSSSSQMS PLSLYGENSN SLSSAEPLKE DKPVTGPRNK TENGLTPKKK IQVNSKPSIQ PKPLLLPAAP KTQ
Target Information
ATF6 is a transcription factor that acts during endoplasmic reticulum (ER) stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) and ERSE II. Binding to ERSE requires NF-Y interaction.
ATF6 forms homo- and heterodimers with ATF6 beta and interacts with transcription factors such as NF-Y, GTF2I, YY1, and SRF. Under ER stress, the cleaved N-terminal domain translocates into the nucleus, functioning as a transcription factor.
ATF6 is N-glycosylated, phosphorylated by MAPK14/P38MAPK, and belongs to the bZIP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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