Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (AER314)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:400

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 4 publications 4 publications

Gel Shift (GS)

1 mg/mL

View 2 publications 2 publications

Product Specifications

Published species

Bovine, Hamster, Human, Mouse

Host/Isotype

Mouse / IgG1, lambda

Class

Monoclonal

Type

Antibody

Clone

AER314

Immunogen

Purified, SDS-denatured calf uterus estrogen receptor (ER) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Estrogen Receptor alpha,uniProtId:P49884-1,ncbiNodeId:9913,antigenRange:1-596,antigenLength:596,antigenImageFileName:MA5-12328_Estrogen_Receptor_alpha_P49884-1_House_mouse.svg,antigenImageFileNamePDP:MA5-12328_Estrogen_Receptor_alpha_P49884-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10984048

Product Specific Information

MA5-12328 targets Estrogen Receptor in WB, GS, and IP applications and shows reactivity with bovine, human, mouse, rabbit, and rat samples. By Western blot, MA5-12328 detects recombinant ER alpha, but may not be suitable for detection of endogenous protein.

The MA5-12328 immunogen is purified, SDS-denatured calf uterus estrogen receptor (ER).

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.