Thermo Fisher Scientific Nestin Monoclonal Antibody (11)
인간 Nestin 단백질을 인식하는 Mouse IgG2a 단클론 항체로, WB, IHC, Flow, IP 등에 적합. 발달 중추신경계 및 줄기세포 마커 연구에 활용. 단백질 A 정제, PBS 버퍼, 무보존제, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | 1–5 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11 |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human Nestin/NES |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898826 |
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
Nestin serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. It is also used as a superior angiogenic marker for evaluating neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin can help characterize progenitor cells differentiating into distinct lineages, enhance therapeutic potential of human neural stem/progenitor cells in CNS disease or injury treatment, and identify neuroepithelial tumor cells.
Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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