Thermo Fisher Scientific GPD2 Polyclonal Antibody
Rabbit polyclonal antibody against human GPD2. Validated for WB and IHC-P applications. Detects endogenous GPD2 protein. Purified by antigen affinity chromatography. Supplied in PBS with glycerol, for research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:20–1:100 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide of human GPD2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2636782 |
Product Specific Information
This antibody detects endogenous levels of total GPD2 protein.
Target Information
Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD.
Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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