Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight 800 4X PEG
DYKDDDDK(FALG) 태그 단백질 검출용 단일클론 항체. DyLight 800 4X PEG 형광 표지로 고감도 Western blot, 형광 이미징에 적합. 마우스 IgG1 항체, Protein A 정제, 4°C 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 4 publications |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| ELISA | – | View 1 publication |
| Immunoprecipitation (IP) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FG4R |
| Immunogen | Synthetic peptide (DYKDDDDK) coupled to KLH |
| Conjugate | DyLight™ 800 4X PEG |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | MES with proprietary stabilizer |
| Contains | No preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537625 |
Additional Formats Available
Product Specific Information
MA1-91878-D800 detects FLAG tag from various samples and has been successfully used for fluorescent detection of FLAG-tagged proteins in Western blotting.
It recognizes a similar epitope as the FLAG tag from Sigma.
DyLight 800 4X PEG has an excitation/emission of 783/797 nm.
Target Information
The DYKDDDDK tag (FLAG tag) is a short peptide sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) commonly used as a protein tag for purification, detection, and localization.
It is typically fused to the N- or C-terminus of a protein, enabling easy identification and isolation using anti-FLAG antibodies.
This tag is widely employed in molecular biology and biochemistry research, including protein-protein interaction studies, protein expression analysis, and localization assays.
The FLAG tag is hydrophilic, minimizing interference with protein folding and activity.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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