Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), NovaFluor Blue 530, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), NovaFluor Blue 530

Applications

• Flow Cytometry (Flow)

Tested Dilution: 4 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-30S
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 660-40S
• NovaFluor Blue 690
• NovaFluor Blue 725
• NovaFluor Blue 760
• NovaFluor Blue 800
• NovaFluor Red 660
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• NovaFluor Yellow 810
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation available

Product Specific Information

The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells.
Recommended usage: 4 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Add 5 µL per sample when staining with NovaFluor conjugates, including single-color controls.

Excitation: 509 nm
Emission: 530 nm
Laser: 488 nm (Blue)

NovaFluor conjugates are based on Phiton™ technology, utilizing novel nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread impact.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) expressed in all nucleated hematopoietic cells, comprising ~10% of surface proteins in lymphocytes. Absent in non-hematopoietic cells and tissues. CD45 regulates Src-family kinases, T/B-cell receptor signaling, cytokine receptor pathways, and cell survival mechanisms. Multiple isoforms arise from alternative splicing with differing extracellular domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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