Thermo Fisher Scientific Villin Polyclonal Antibody
Western blot 및 ICC/IF에 적합한 Rabbit Polyclonal 항체로, Human 및 Mouse Villin 단백질을 검출. 고순도의 항원 친화 크로마토그래피 정제 제품. PBS 기반 완충액에 1% BSA와 20% 글리세롤 포함. 위장관 및 신장 상피 세포 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Bovine, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human Villin (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736783 |
Product Specific Information
- Positive Control: HepG2, mouse kidney
- Predicted Reactivity: Rat (87%), Bovine (87%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Villin is a cytoskeletal protein associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It interacts with actin in a Ca²⁺ and phosphoinositide-regulated manner. Villin serves as a specific marker for adenocarcinomas of the gastrointestinal tract and pancreas, and is also expressed in some Merkel cell tumors and adenocarcinomas of lung, prostate, ovarian, and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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