Thermo Fisher Scientific VEGF-D Human ProcartaPlex Simplex Kit

Invitrogen™ VEGF-D Human ProcartaPlex™ Simplex Kit

The Human VEGF-D Simplex ProcartaPlex Kit measures VEGF-D protein and is designed to be combinable with other Simplex kits to create a customized multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits (not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is required per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate multiple protein targets simultaneously using a Luminex instrument.

• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX). Similar to a sandwich ELISA, the assay uses matched antibody pairs to identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).

Unlike traditional ELISA, the capture antibody in ProcartaPlex assays is conjugated to a bead rather than adsorbed to a microplate well, allowing reagents to remain free-floating in solution. Detection is achieved using two lasers: one to distinguish bead signatures and another to quantify RPE fluorescence, proportional to the protein amount. ProcartaPlex assays enable profiling of more targets with less sample volume in the same time as a conventional ELISA.

ProcartaPlex multiplex panels are available for six species (human, mouse, rat, nonhuman primate, porcine, canine).
For more information and a complete target list, visit thermofisher.com/procartaplex.

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