Thermo Fisher Scientific CarboxyLink Immobilization Kit, 2 mL

Thermo Fisher Scientific CarboxyLink™ Immobilization Kit, 2 mL

The Thermo Scientific™ CarboxyLink™ Immobilization Kit enables covalent immobilization of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

CarboxyLink Resin is a crosslinked beaded agarose activated with diamino-dipropylamine (DADPA), providing long spacer arms each with a primary amine at the end. When incubated with the carbodiimide crosslinker EDC (included in the kit), carboxyl-containing molecules become permanently attached to the support by stable amide bonds. Once coupled, the prepared affinity column can be reused multiple times for protein affinity purification. The resin can also be used for immobilizing other molecules via alternative amine-reactive chemistries.

Features

• CarboxyLink Coupling Resin: DADPA-activated crosslinked 4% beaded agarose
• Efficient immobilization: Couple 1–2 mg of peptide per mL of resin
• Stable linkage: Covalent amide bond attachment enables multiple purification cycles
• Flexible and gentle conditions: Reaction in MES or other non-amine, near-neutral buffers
• Ideal for unmodified peptides: High capacity and minimal steric hindrance
• Convenient kit options: Includes buffers, crosslinker, and spin/drip columns

Application Notes

Because EDC crosslinks carboxylates to primary amines, peptides containing both functional groups may polymerize as well as immobilize. For peptides with terminal carboxyl and amino groups, coupling results in end-to-end linkage providing high capacity and low steric hindrance. Peptides with internal lysine, glutamic, or aspartic residues may experience blocked binding sites, reducing affinity capacity.

Alternative immobilization methods:

• AminoLink Plus Coupling Resin
• UltraLink Biosupport
• SulfoLink Coupling Resin

Related Products

• CarboxyLink™ Coupling Resin
• CarboxyLink™ Immobilization Kit with UltraLink™ Support, 2 mL

Specifications