Invitrogen™

Total Exosome RNA & Protein Isolation Kit

The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from자세히 알아보기

The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum)).
• Extracts highly pure total RNA (including small RNA fraction) from exosomes
• Recovers protein and RNA from the same sample
• Compatible with all protocols for exosome isolation

The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). It is suitable for studies of RNA expression (specifically miRNA), processing, or function. The kit allows recovery of both RNA and proteins from the same purified exosome preparation. One portion of the sample is subjected to organic extraction, followed by immobilization of RNA on glass-fiber filters to purify total RNA or enrich for small RNA species. The other portion is then used directly for common protein applications such as Western blotting.

High yields of ultra-pure RNA can be prepared in about 30–60 minutes. The RNA can then be used in a variety of downstream applications, including qRT-PCR, high-throughput sequencing (e.g., using the Ion Torent™ PGM™, Ion Torrent™ Proton™, or SOLiD™ sequencing instruments), microarray analysis, solution hybridization assays, and blot hybridization.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

사양

용도(애플리케이션)Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Western Blot, microRNA Analysis

제품 유형Total Exosome Isolation

최종 제품 유형Total RNA, Protein, micro RNA

고처리량 호환성Not High-throughput Compatible (Manual)

분리 기술Spin Column (Glass Fiber Filter), Organic Extraction

정제 타겟Total RNA, Protein, RNA & Protein, miRNA

수량40 preps

샘플 종류Organelle & Vesicle-Related Samples, Exosomes

배송 조건Room Temperature

Unit SizeEach