Thermo Fisher Scientific Phospho-PKC delta (Ser645) Polyclonal Antibody
Phospho-PKC delta (Ser645) 폴리클로날 항체로, 인간, 마우스, 랫트 시료에서 PKCδ 인산화 상태 검출에 적합합니다. WB, IHC, ICC, Flow, ELISA 등 다양한 응용 가능. Protein A로 정제된 고순도 항체로, -20°C 보관 안정성 우수.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human PKC delta around the phosphorylation site of Ser645 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified, differing in structure, tissue distribution, localization, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family, which are Ca²⁺- and phospholipid-dependent. PKC family members phosphorylate diverse protein targets and participate in various signaling pathways, serving as major receptors for phorbol esters. PKC delta is involved in B cell signaling and regulation of growth, apoptosis, and differentiation in multiple cell types. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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