Thermo Fisher Scientific ATG4B Monoclonal Antibody (OTI1A3), TrueMAB
ATG4B 단백질을 인식하는 Mouse IgG2b 단일클론 항체로 Western blot 및 IHC(P) 실험에 적합. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제. PBS/BSA/glycerol 완충액에 보관, -20°C에서 안정적 저장.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A3 |
| Immunogen | Full-length human recombinant ATG4B protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cysteine protease ATG4B is an enzyme encoded by the ATG4B gene. Autophagy is a critical cellular homeostatic process that regulates the turnover of damaged organelles and proteins.
ATG4B acts as a deubiquitin-like protease with dual roles in the autophagy core machinery. It cleaves the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, GABARAP) to expose a C-terminal glycine, which is essential for conjugation to phosphatidylethanolamine (PE) and membrane insertion.
ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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