
Thermo Fisher Scientific IPTG, dioxane-free, 5 g
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Thermo Scientific™
IPTG, dioxane-free
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis자세히 알아보기
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon (see Reference 1). It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.
Applications
• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (see Reference 1)
• Induction of gene expression under control of the lac promoter
Note
• Preparation of a 100 mM (23.83 mg/mL) stock solution in water is recommended
• For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media
• IPTG, dioxane-free, can be stored at +4°C
Related Products
IPTG Solution, ready-to-use
IPTG, dioxane-free
사양
화학물질 이름 또는 재질Isopropylthiogalactoside
농도0.1 mM
제품 유형IPTG Solution
수량5 g
형태Powder
Unit SizeEach
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