Thermo Fisher Scientific Rabbit anti-Bovine IgG (H+L) Secondary Antibody, AP
토끼에서 유래한 항-소 IgG (H+L) 폴리클로날 2차 항체로, AP 결합형입니다. Western blot, IHC, ELISA 등 다양한 응용에 사용 가능하며, 높은 특이성과 민감도를 제공합니다. 친화 크로마토그래피로 정제되어 신뢰성 높은 결과를 보장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Bovine IgG (H+L) |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535912 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Biotin conjugate
- FITC conjugate
- HRP conjugate
- Texas Red conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Bovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for bovine (cow, ox) immunoglobulins. They are useful in the detection, sorting, or purification of their specified targets. Secondary antibodies offer increased versatility by enabling the use of various detection systems (e.g., HRP, AP, fluorescence) and can enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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