Takara Protein digestion: arginylendopeptidase

More Information

Applications

• Protein digestion
• Fragmentation of proteins and peptides prior to structural analysis

Source

Mouse submaxillary gland

Storage

−20°C

Properties

Form

In a solution of 5 mM sodium phosphate buffer (pH 7.2) containing 50% glycerol.

Definition of activity

One unit of activity corresponds to the amount of enzyme required to produce 1 mmol p-nitroaniline from benzoyl-DL-arginine p-nitroanilide (BAPA) in 1 min at 37°C and pH 8.0.

Product Citations

Isackson PJ, Silverman RE, Blaber M, Server AC, Nichols RA, Shooter EM, Bradshaw, R. Epidermal growth factor binding protein: identification of a different protein. Biochemistry 26, 2082-5 (1987).

Matsushita H, Kato I, Aoyama H, Tsunasawa S, S. F. Arginylendopeptidase. Protein, Nucleic Acid Enzym. (Japanese Journal) 34, 374-9 (1989).

Schenkein, I., Levy, M., Franklin, E.C., Frangione, B. Proteolytic enzymes from the mouse submaxillary gland. Specificity restricted to arginine residues. Arch Biochem Biophys. 182, 64-70 (1977).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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