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Takara Protein digestion: arginylendopeptidase
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Takara Protein digestion: arginylendopeptidase

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단백질 및 펩타이드 절단에 적합한 Arginylendopeptidase 효소. Mouse submaxillary gland 유래로 구조 분석 전 단백질 절편화에 사용. 21.3 kDa 분자량, pH 8.0–9.0 최적. −20°C에서 보관하며, PMSF 및 DFP에 의해 억제됨.

카탈로그번호
7308
브랜드
Takara
카테고리
Enzymes
판매단위
pk
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Takara 7308 Arginylendopeptidase, 0.5 mg pk판매 단위 pk ·
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Takara · Takara Protein digestion: arginylendopeptidase

Protein Digestion: Arginylendopeptidase

Applications

  • Protein digestion
  • Fragmentation of proteins and peptides prior to structural analysis

Source

Mouse submaxillary gland

Storage

−20°C

Properties

항목 내용
Molecular weight 21.3 kDa (gel filtration)
Optimum pH 8.0–9.0
Isoelectric point 5.65
Inhibitors PMSF, DFP
Tolerance to denaturants ≤ 2 M Urea
≤ 0.1 M Guanidine-HCl
≤ 0.05% SDS

Form

5 mM sodium phosphate buffer (pH 7.2) containing 50% glycerol 용액 상태.

Definition of Activity

One unit of activity corresponds to the amount of enzyme required to produce 1 mmol p-nitroaniline from benzoyl-DL-arginine p-nitroanilide (BAPA) in 1 min at 37°C and pH 8.0.

Product Citations

  • Isackson PJ, Silverman RE, Blaber M, Server AC, Nichols RA, Shooter EM, Bradshaw R. Biochemistry 26, 2082–2085 (1987).
  • Matsushita H, Kato I, Aoyama H, Tsunasawa S, S. F. Protein, Nucleic Acid Enzym. (Japanese Journal) 34, 374–379 (1989).
  • Schenkein I, Levy M, Franklin EC, Frangione B. Arch Biochem Biophys. 182, 64–70 (1977).

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components List and Certificates of Analysis are available under the Documents tab.


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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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