Thermo Fisher Scientific GlycoLink Immobilization Kit
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Thermo Fisher Scientific GlycoLink™ Immobilization Kit
The Thermo Scientific™ GlycoLink™ Immobilization Kit provides affinity columns and reagents designed to immobilize glycoproteins through oxidized sugar groups for use in affinity purification or protein–protein interaction studies.
Features
- High capacity: Immobilize 1–10 mg of oxidized antibody or glycoprotein per mL of resin
- Fast and efficient: Complete immobilization in as few as 30 minutes; achieve ≥90% coupling in under 4 hours
- Specific immobilization: Hydrazide-activated UltraLink™ Resin reacts only with oxidized glycoproteins
- Stable attachment: Hydrazone bonds are stable for multiple reuse cycles without additional stabilization
- Preserves antibody function: Coupling via Fc region retains both antigen-binding sites
Description
The kit uses hydrazide-activated resin and a coupling catalyst to prepare affinity columns with polyclonal or monoclonal antibodies containing periodate-oxidizable carbohydrate groups. The resulting immobilized antibodies maintain unobstructed antigen-binding sites, enabling optimal purification performance.
Prepared columns can be regenerated and reused at least five times without significant loss in binding capacity.
Applications
- Purification of antigens using covalently coupled antibodies
- Identification of binding partners for target glycoproteins
- Large-scale affinity purification
Principle of Operation
Glycoproteins, such as antibodies, contain carbohydrate moieties with cis-diol groups that can be oxidized by sodium meta-periodate to form aldehydes. These aldehydes react with hydrazide groups on the resin to form stable covalent bonds. The GlycoLink Coupling Catalyst accelerates this reaction, enhancing coupling efficiency and reducing incubation time.
Typical coupling reactions occur within 2–4 hours in a non-amine buffer, achieving >85% efficiency and yielding 1–10 mg of immobilized protein per mL of UltraLink™ Hydrazide Resin.
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Specifications
| 항목 | 내용 |
|---|---|
| 설명 | GlycoLink™ Immobilization Kit |
| 제품라인 | GlycoLink™ |
| 유형 | Immobilization Kit |
| 컬럼 유형 | Affinity, UltraLink™ Resin |
| 정지상 | Hydrazide |
| 형식 | Spin Column |
| 용량(미터법) | 1–10 mg of Glycoprotein Immobilization per Column |
| 수량 | 10 Columns Kit |
| 충분 | 10 Columns |
| Unit Size | Each |
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