Thermo Fisher Scientific GlycoLink Immobilization Kit

Thermo Fisher Scientific GlycoLink™ Immobilization Kit

The Thermo Scientific™ GlycoLink™ Immobilization Kit provides affinity columns and reagents designed to immobilize glycoproteins through oxidized sugar groups for use in affinity purification or protein–protein interaction studies.

Features

• High capacity: Immobilize 1–10 mg of oxidized antibody or glycoprotein per mL of resin
• Fast and efficient: Complete immobilization in as few as 30 minutes; achieve ≥90% coupling in under 4 hours
• Specific immobilization: Hydrazide-activated UltraLink™ Resin reacts only with oxidized glycoproteins
• Stable attachment: Hydrazone bonds are stable for multiple reuse cycles without additional stabilization
• Preserves antibody function: Coupling via Fc region retains both antigen-binding sites

Description

The kit uses hydrazide-activated resin and a coupling catalyst to prepare affinity columns with polyclonal or monoclonal antibodies containing periodate-oxidizable carbohydrate groups. The resulting immobilized antibodies maintain unobstructed antigen-binding sites, enabling optimal purification performance.
Prepared columns can be regenerated and reused at least five times without significant loss in binding capacity.

Applications

• Purification of antigens using covalently coupled antibodies
• Identification of binding partners for target glycoproteins
• Large-scale affinity purification

Principle of Operation

Glycoproteins, such as antibodies, contain carbohydrate moieties with cis-diol groups that can be oxidized by sodium meta-periodate to form aldehydes. These aldehydes react with hydrazide groups on the resin to form stable covalent bonds. The GlycoLink Coupling Catalyst accelerates this reaction, enhancing coupling efficiency and reducing incubation time.
Typical coupling reactions occur within 2–4 hours in a non-amine buffer, achieving >85% efficiency and yielding 1–10 mg of immobilized protein per mL of UltraLink™ Hydrazide Resin.

More Product Data

• Improved immobilization and conjugation of glycoproteins

Related Products

• GlycoLink™ Micro Immobilization Kit (Cat. No. 88942)
• UltraLink™ Hydrazide Resin (Cat. No. 53149)

Specifications

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