Thermo Fisher Scientific EIF4E2 Monoclonal Antibody (OTI1F2), TrueMAB
EIF4E2 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, Flow cytometry에 적합. 고순도 친화 크로마토그래피 정제, 액상 형태로 제공되며 안정적인 PBS/BSA/glycerol 버퍼에 보관. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F2 |
| Immunogen | Full length human recombinant protein of human EIF4E2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.66 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349). Acts as a repressor of translation initiation (PubMed:22751931).
In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2, or EIF4G3), suggesting that it acts by competing with EIF4E and blocks assembly of eIF4F at the cap. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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