Thermo Fisher Scientific Amplex Red Uric Acid/Uricase Assay Kit

Invitrogen™ Amplex™ Red Uric Acid/Uricase Assay Kit

The Amplex™ Red Uric Acid/Uricase Assay Kit provides a sensitive and simple method for detecting uric acid or uricase using either a fluorescence microplate reader or fluorometer.

• Detect concentrations as low as 100 nM uric acid or 0.2 mU/mL of uricase activity
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference

See our complete line of Fluorescence Microplate assays

Background

Serum uric acid is the end product of purine metabolism in body tissues and is cleared through the kidneys by glomerular filtration. Most animals can metabolize uric acid to more readily excreted products, but humans lack the necessary enzyme urate oxidase (uricase) due to two nonsense mutations in the human gene for uricase.

Principle of the Assay

The Amplex™ Red Uric Acid/Uricase Assay Kit provides an ultrasensitive method for detecting uric acid or monitoring uricase activity.
In the assay:

1. Uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide, and carbon dioxide.
2. In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts stoichiometrically with the Amplex™ Red reagent to generate the red-fluorescent oxidation product resorufin.

Resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, minimizing interference from autofluorescence in most biological samples.

Applications

Use Amplex™ Red Assays for a broad range of investigations, including:

• Cell signaling and lipid metabolism
• Neurobiology
• Inflammation and immune function
• Metabolic studies

Additional related reagents:

• Amplex™ UltraRed Reagent (Cat. No. A36006) — second-generation reagent with greater sensitivity and brighter fluorescence
• Amplex™ Red/UltraRed Stop Reagent (Cat. No. A33855) — allows termination of fluorescence reaction at a user-defined time point; signal remains stable for at least three hours

Custom assay design and packaging options are also available.

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