Thermo Fisher Scientific KIAA1524 Monoclonal Antibody (OTI1C11), TrueMAB
KIAA1524(CIP2A) 단백질을 인식하는 마우스 단클론 항체로 Western blot 및 IHC(P) 검증 완료. 정제된 항체로 동결건조 형태 제공. 인산화 단백질 연구 및 암 관련 신호전달 연구에 적합. -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 635–905 of human KIAA1524 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Inhibition of protein phosphatase 2A (PP2A) activity is essential for human cell transformation. The protein Cancerous Inhibitor of PP2A (CIP2A) interacts with the oncogenic transcription factor c-Myc, inhibits PP2A activity toward c-Myc serine 62 (S62), and prevents c-Myc proteolytic degradation.
CIP2A stabilizes c-Myc, promotes anchorage-independent cell growth, and supports in vivo tumor formation. Overexpression of CIP2A induces oncogenic transformation and is observed in head and neck squamous cell carcinoma (HNSCC) and colon cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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