Thermo Fisher Scientific eIF4G1/eIF4GI Polyclonal Antibody
Human eIF4G1 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, IP 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 액상 형태로 제공. 연구용으로 mRNA 번역 개시 복합체 관련 단백질 분석에 적합.
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Thermo Fisher Scientific eIF4G1/eIF4GI Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 200 and 250 of human eukaryotic translation initiation factor 4 gamma, 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The protein encoded by this gene is a component of the EIF4F complex, involved in mRNA cap recognition, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome.
Alternative splicing results in five transcript variants encoding four distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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