Thermo Fisher Scientific CDKN2A/p14ARF Polyclonal Antibody, Biotin
CDKN2A/p14ARF 단백질을 인식하는 Biotin 결합 토끼 폴리클로날 항체. Western blot, IHC, ELISA, IP 등 다양한 응용에 사용 가능. 인간, 마우스, 설치류, 영장류 시료에 반응. 세포주기 조절 및 종양 억제 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-epitope synthetic peptide corresponding to unique amino acid sequence on p14ARF protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and p19ARF in mouse). These two proteins interact with the upstream tumor suppressors, retinoblastoma protein and p53, respectively. Each protein contributes to primary cell senescence, cell cycle regulation, and tumor suppression. p14ARF (or p19ARF in mouse) induces G1 and G2 phase arrest in a p53-dependent manner. Functional differences between mouse and human ARF proteins may underlie species-specific tumor susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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