Thermo Fisher Scientific Phospho-c-kit (Tyr936) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). It was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT and its ligand regulate the growth and activation of various hematopoietic and non-hematopoietic cells.

Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of KIT receptor tyrosine kinase, particularly the D816V activation loop mutation, is a therapeutic target for neoplastic mast cell growth.

c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells. Upon binding to stem cell factor (SCF), c-Kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues (including Tyr703). This enables Grb2 docking and activation of the Ras/ERK signaling pathway. SCF/c-Kit signaling also activates PI3K, PLC-γ, and JAK/STAT pathways, essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.