Thermo Fisher Scientific PAX8 Polyclonal Antibody
PAX8 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보존. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1–204 (Q06710) of PAX8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.84 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_11151941 |
Product Specific Information
- Recommended positive controls: 293T, 293T nuclear extract, DDDDK-tagged PAX8-transfected 293T
- Predicted reactivity: Mouse (98%), Rat (99%), Xenopus laevis (91%), Dog (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The PAX8 gene encodes a member of the paired box family of transcription factors. These proteins typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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