Thermo Fisher Scientific DNAJB2 Polyclonal Antibody
Rabbit polyclonal antibody targeting DNAJB2, validated for WB and IHC applications. Reacts with human and predicted cross-reactivity to multiple species. Supplied unconjugated in liquid form, purified by antigen affinity chromatography. Ideal for neuro...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–238 of DNAJB2 (Uniprot ID: P25686) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris Glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153085 |
Product Specific Information
- Recommended positive control: A431
- Predicted reactivity: Mouse (91%), Rat (83%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
The DnaJ family is one of the largest chaperone families, with diverse cellular localizations and functions. The defining J domain characterizes DnaJ proteins, which interact with HSP70 to stimulate ATP hydrolysis. These proteins contain cysteine-rich zinc finger regions forming peptide-binding domains crucial for chaperone activity. DnaJ proteins are involved in proteolysis, protein degradation regulation, exocytosis, and endocytosis.
DnaJB2 (DnaJ homolog subfamily B member 2), also known as HSJ1 or HSPF3, is expressed almost exclusively in the brain, particularly in the frontal cortex and hippocampus. Two isoforms are produced via alternative splicing.
⚠ WARNING: This product contains chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm. For details, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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