Thermo Fisher Scientific Intracellular pH Calibration Buffer Kit

Invitrogen™ Intracellular pH Calibration Buffer Kit

The Intracellular pH Calibration Buffer Kit enables the quantitation of intracellular pH when used in conjunction with pHrodo™ Red AM, pHrodo™ Green AM, or BCECF intracellular pH indicators.

pHrodo™ Red AM and pHrodo™ Green AM are weakly fluorescent at neutral pH but become increasingly fluorescent as pH decreases. The dyes are modified with AM ester groups to allow cell membrane permeability. Once inside the cell, the ester groups are cleaved by nonspecific esterases, trapping the dye intracellularly. The resulting fluorescence intensity reflects intracellular pH.

Subsequent use of the Intracellular pH Calibration Buffer Kit allows this intracellular pH to be quantified.

Kit Description

The kit contains a range of pH calibration buffers (pH 4.5, 5.5, 6.5, and 7.5) and includes valinomycin and nigericin, which equilibrate intra- and extracellular pH.
After measuring fluorescence intensity under experimental conditions using pHrodo™ Red AM or pHrodo™ Green AM, cells are resuspended in each calibration buffer containing valinomycin and nigericin. Fluorescence intensity is measured for each buffer to generate a standard curve, enabling accurate intracellular pH determination.

사양 (Specifications)