Thermo Fisher Scientific Amplex Red Phospholipase D Assay Kit

Invitrogen™ Amplex™ Red Phospholipase D Assay Kit

The Amplex™ Red Phospholipase D Assay Kit provides a sensitive and simple method to detect Phospholipase D (PLD) activity using a fluorescence microplate reader or fluorometer.

• Detect phospholipase D activity levels as low as 10 U/mL
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference

PLD activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex™ Red reagent), a sensitive fluorogenic probe for hydrogen peroxide.
PLD converts phosphatidylcholine (lecithin) to choline, which is then oxidized by choline oxidase to betaine and hydrogen peroxide.
In the presence of horseradish peroxidase, hydrogen peroxide reacts with the Amplex™ Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin.

Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.

Use Amplex™ Red Assays for a Broad Range of Investigations

A wide variety of validated Amplex™ Red assays are available for studying:

• Cell signaling and lipids
• Neurobiology
• Inflammation and immune function
• Metabolism

We also offer:

• Amplex™ UltraRed Reagent (Cat. No. A36006) – Second-generation reagent providing greater sensitivity and brighter fluorescence
• Amplex™ Red/UltraRed Stop Reagent (Cat. No. A33855) – Allows termination of fluorescence generation at desired time points; signal remains stable for at least three hours

Custom assay design and packaging are also available.

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