Thermo Fisher Scientific Phospho-PLCG1 (Tyr1253) Polyclonal Antibody
Phospho-PLCG1 (Tyr1253) 항체로 인간, 마우스, 랫트 시료에서 인산화 PLCG1 검출에 적합. Western blot에 1:500~1:1,000 희석 사용. 항원 친화 크로마토그래피로 정제된 rabbit polyclonal IgG. -20°C 보관, 연구용 전용.
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Applications and Tested Dilution
- Western Blot (WB): 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphopeptide derived from human PLCG1 around the phosphorylation site of Tyr1253 (S-R-YP-Q-Q) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco’s PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2554919 |
Target Information
The mammalian Phospholipase Cγ (PLCγ) family includes two closely related proteins, PLCγ1 and PLCγ2. These isozymes share core domain structures and possess additional domains: one PH domain, two SH2 domains, and one SH3 domain. Upon extracellular stimulation by hormones or growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCγ. Activated PLCγ hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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