Thermo Fisher Scientific TFAM Polyclonal Antibody
Rabbit polyclonal antibody against human TFAM for WB, IHC, IHC-P, and IP applications. Recognizes residues 196–246 of mitochondrial transcription factor A. Supplied as unconjugated liquid at 1 mg/mL, stored at 4°C. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 196 and 246 of Human Transcription Factor A, Mitochondrial |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
Target Information
Mitochondrial transcription factor A (TFAM) is a nuclear-encoded protein that translocates to mitochondria, where it maintains and regulates mitochondrial DNA (mtDNA) replication and transcription. TFAM is essential for mitochondrial genome packaging, stability, and expression. It contains an LC3 interacting region (LIR) motif, enabling interaction with autophagy proteins such as LC3, crucial for the autolysosomal pathway that removes leaked mtDNA and reduces inflammation. TFAM levels vary by tissue and influence mitochondrial function and oxidative phosphorylation (OXPHOS). Dysregulation of TFAM can lead to mitochondrial dysfunction and diseases associated with mtDNA stress, including neurodegeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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