Thermo Scientific™

Klenow Fragment, exo– (5 U/μL)

Thermo Scientific Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5→3 polymerase activity, but자세히 알아보기

Thermo Scientific Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5→3 polymerase activity, but lacks the 3→5 and 5→3 exonuclease activities of DNA Polymerase I. The 3→5 exonuclease activity of the enzyme is eliminated by mutations in the 3→5-exonuclease active site.

Highlights

• Lacks 3→5 exonuclease activity
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
• Active in restriction enzyme, PCR, and RT buffers

Applications
• Random-primed DNA labeling
• Labeling by fill-in 5`-overhangs of dsDNA
• Strand displacement amplification (SDA)
• DNA sequencing by the Sanger method

Note

Klenow Fragment, exo- is not recommended for DNA blunting reactions prior to DNA ligation since it frequently adds one or more extra nucleotides to the 3`-terminus of blunt-end DNA substrates in a non-template directed fashion.

사양

농도5 U/μL

중합효소DNA Polymerase I

함께 사용가능한 버퍼Restriction Enzyme, PCR, RT Buffer

수량300 units

제품 유형Klenow Fragment

Unit SizeEach