Thermo Fisher Scientific Phospho-LCK (Tyr505) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

LCK is a member of the Src-family tyrosine kinases, essential for signaling through the T-cell receptor (TCR) complex. Upon TCR activation, LCK phosphorylates ITAM motifs in the zeta subunits, creating binding sites for SH2 domains of tyrosine kinase ZAP70. ZAP70 is further phosphorylated and activated by LCK, leading to downstream signaling via adaptor LAT.
Most LCK localizes to the plasma membrane, but a fraction associates with the Golgi apparatus, potentially contributing to Raf activation under weak TCR stimulation. LCK features N-terminal myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs.
LCK also regulates apoptosis induced by various stimuli, excluding those mediated by death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternative splice variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.