Thermo Fisher Scientific BTF3L4 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen, and the purity is >95% (by SDS-PAGE).

Target Information

The initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements such as the TATA box. In addition to RNA polymerase II (Pol II), the transcription factor class II (TFII) family of proteins are required for initiation of transcription, as the first step in the formation of this initiation complex is the stable binding of TFIID to the TATA box.

An additional TFII-related protein, BTF3, forms a stable complex with Pol II and facilitates its assembly. The BTF3 gene encodes two isoforms, BTF3a and BTF3b, differing by the absence of 44 amino acids at the N-terminal of BTF3b. This deletion prevents BTF3b from inducing transcription, although it can still bind Pol II.

Both isoforms associate with casein kinase II, which phosphorylates BTF3a and TFIIB, contributing to efficient transcription of tRNA and 5S rRNA genes by Pol III. BTF3 belongs to the NAC-beta family, which includes BTF3L1, BTF3L2, BTF3L3, and BTF3L4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.