Thermo Fisher Scientific GEMIN8 Monoclonal Antibody (OTI1E5)
GEMIN8 단백질을 표적으로 하는 Mouse monoclonal 항체로 Western blot 및 IHC(P) 검증 완료. Affinity chromatography로 정제된 1 mg/mL 액상 제품이며, PBS 기반 버퍼에 보존. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E5 |
| Immunogen | Full length human recombinant protein of GEMIN8 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724360 |
Target Information
The SMN complex plays a catalytic role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. It is essential for the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins (SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, and SNRPG) that form a heptameric ring on the Sm site of small nuclear RNA.
In the cytosol, Sm proteins are initially trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A. The SMN complex dissociates CLNS1A and transfers Sm proteins to the SMN-Sm complex, triggering core snRNP assembly and nuclear transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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